PCR Analysis of Tail DNA: Difference between revisions

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 ==Protocol==
-Use the following Volumes per Reaction:
+Use the following Volumes per 50ul Reaction:
-#Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)
+#10X GoTaq Buffer: 5uL ("Molecular Biology Stuff" box in freezer)
-#Forward Primer: 0.4ul
+#Primer Mix: 5ul
-#Reverse Primer: 0.4ul
+#dNTPs: 0.5uL of 10 mM ("Molecular Biology Stuff" box in freezer)
-#dNTPs: 0.4uL of 2 mM ("Molecular Biology Stuff" box in freezer)
+#Sterile water: 29ul
-#Sterile water: 13.6 uL
+#Polymerase Go-Taq: 0.125uL ("Molecular Biology Stuff"  box in freezer)
-#Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)
 #Template: 1 uL
+Master Mix
+#10X GoTaq Buffer: 50uL ("Molecular Biology Stuff" box in freezer)
+#Primer Mix: 50ul
+#dNTPs: 5uL of 10 mM ("Molecular Biology Stuff" box in freezer)
+#Sterile water: 290ul
+#Polymerase Go-Taq: 1.25ul ("Molecular Biology Stuff"  box in freezer)
+*Add Template Individually
 Run PCR Program (approx 2 hours).