Difference between revisions of "Genotyping Program"

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(added gdf15)
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|[[ppp1r3c Genotyping| PTG]] || ppp1r3c (PTG) || KO- 574bp, WT- 678bp || Two bands if heterozygote (run primers separately)
 
|[[ppp1r3c Genotyping| PTG]] || ppp1r3c (PTG) || KO- 574bp, WT- 678bp || Two bands if heterozygote (run primers separately)
 
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|[[4EBP1/2 Genotyping| 4EBP1/2]] || [[Cre PCR]] || 1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp || Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
+
|[[4EBP1/2 Genotyping| 4EBP1/2]] || [[Cre PCR]] || 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp || Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
 
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|[[GDF15 Genotyping| GDF15]] || GDF15 || KO- 200bp, WT- 600bp || Two bands if heterozygote (run primers separately)
 
|[[GDF15 Genotyping| GDF15]] || GDF15 || KO- 200bp, WT- 600bp || Two bands if heterozygote (run primers separately)

Revision as of 14:47, 8 June 2020

Allele Program Fragment Size(s) Notes
TSC-floxed Cre PCR KO- 368bp, WT- 295bp Two bands if heterozygote
Cre Cre PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Cre PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote
ob/ob Genotyping ob/ob KO - 123bp, WT-104kb, control 191 bp Two reactions, see ob/ob Genotyping
MTM1 MTM1 Mutant-575bp, WT-714bp Separate Reactions for WT and KO
CAP CAP Two bands if heterozygote, upper band for KO
Vinexin Vinexin
GR KO nr3c1 KO- 300bp, WT- 247bp Two bands if heterozygote
mc4r-Cone mc4r KO- 540bp, WT- 400bp Two bands if heterozygote (run primers separately)
PTG ppp1r3c (PTG) KO- 574bp, WT- 678bp Two bands if heterozygote (run primers separately)
4EBP1/2 Cre PCR 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
GDF15 GDF15 KO- 200bp, WT- 600bp Two bands if heterozygote (run primers separately)