Difference between revisions of "Genotyping Program"

Revision as of 14:10, 8 June 2020

Allele	Program	Fragment Size(s)	Notes
TSC-floxed	Cre PCR	KO- 368bp, WT- 295bp	Two bands if heterozygote
Cre	Cre PCR		Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14	Cre PCR		Two bands if heterozygote
TC10b	TC10b	KO - 500bp, WT-1kb	Two bands if heterozygote
ob/ob Genotyping	ob/ob	KO - 123bp, WT-104kb, control 191 bp	Two reactions, see ob/ob Genotyping
MTM1	MTM1	Mutant-575bp, WT-714bp	Separate Reactions for WT and KO
CAP	CAP		Two bands if heterozygote, upper band for KO
Vinexin	Vinexin
GR KO	nr3c1	KO- 300bp, WT- 247bp	Two bands if heterozygote
mc4r-Cone	mc4r	KO- 540bp, WT- 400bp	Two bands if heterozygote (run primers separately)
PTG	ppp1r3c (PTG)	KO- 574bp, WT- 678bp	Two bands if heterozygote (run primers separately)
4EBP1/2	Cre PCR	1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp	Two bands if heterozygote for each allele (run primers for 1 and 2 separately)

@@ Line 20: / Line 20: @@
 |[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || Two bands if heterozygote
 |-
-|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540, WT- 400 || Two bands if heterozygote (run primers separately)
+|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540bp, WT- 400bp || Two bands if heterozygote (run primers separately)
 |-
-|[[ppp1r3c Genotyping| PTG]] || ppp1r3c (PTG) || KO- 574, WT- 678 || Two bands if heterozygote (run primers separately)
+|[[ppp1r3c Genotyping| PTG]] || ppp1r3c (PTG) || KO- 574bp, WT- 678bp || Two bands if heterozygote (run primers separately)
+|-
+|[[4EBP1/2 Genotyping| 4EBP1/2]] || [[Cre PCR]] || 1=KO- 550bp, WT- 175bp; 2=KO- 750bp, WT- 450bp || Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
 |}