Genotyping Program: Difference between revisions

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{| border="1"

!| Allele || Program|| Fragment Size(s) || Notes

!| Allele || Program|| Fragment Size(s) || Strain(s) || Notes

|-

|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || || Two bands if heterozygote

|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || aTSC, mTSC, Placenta TSC || Two bands if heterozygote

|-

|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene)

|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || Between 340-400kDa || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR || Only present if Transgene Positive (cannot detect homozygosity at transgene)

|-

|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]|| || Two bands if heterozygote

|[[SRY Genotyping| SRY]]|| [[Cre PCR ]] || Between 100-200kDa || any mice too young to sex visually || Only present in males

|-

|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]|| || || Two bands if heterozygote

|-

|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote

|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || || Two bands if heterozygote

|-

|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]

|-

|[[~~Ob/ob_Genotyping~~|~~ob/ob Genotyping~~]] || ~~ob/ob~~|| KO - ~~123bp~~, WT-~~104kb, control 191 bp~~ || Two ~~reactions~~, ~~see~~ [[~~Ob/ob_Genotyping~~|~~ob/ob~~ Genotyping]]

|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp || || Separate Reactions for WT and KO

|-

|[[~~MTM1~~ Genotyping|~~MTM1~~]] || ~~MTM1~~ || ~~Mutant~~-~~575~~, WT-~~714~~ || ~~Separate Reactions for~~ WT ~~and KO~~

|[[CAP Genotyping|CAP]] || CAP || || || Two bands if heterozygote, upper band for KO

|-

|[[Vinexin Genotyping|Vinexin]] || Vinexin || || ||

|-

|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || GR-KO and Adipo-GR-KO || Two bands if heterozygote

|-

|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540bp, WT- 400bp || mc4r || Two bands if heterozygote (run primers separately)

|-

|[[ppp1r3c Genotyping| PTG]] || ppp1r3c (PTG) || KO- 574bp, WT- 678bp || ppp1r3c (PTG) || Two bands if heterozygote (run primers separately)

|-

|[[~~CAP~~ Genotyping|~~CAP~~]] || ~~CAP~~ || || Two bands if heterozygote~~, upper band~~ for KO

|[[4EBP1/2 Genotyping| 4EBP1/2]] || [[Cre PCR]] || 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp || 4EBP1/2 || Two bands if heterozygote for each allele (run primers for 1 and 2 separately)

|-

|[[~~Vinexin~~ Genotyping|~~Vinexin~~]] || ~~Vinexin~~ || ||

|[[GDF15 Genotyping| GDF15]] || GDF15 || KO- 200bp, WT- 600bp || GDF15 || Two bands if heterozygote (run primers separately)

|}

AMPK, fTSC, fRaptor, and C57Bl6/J are all wild-type mice and don't need to be genotyped.

[[ Category:Genotyping ]]

[[ Category:Mouse Work]]

@@ Line 1: / Line 1: @@
 {| border="1"
-!| Allele || Program|| Fragment Size(s) || Notes
+!| Allele || Program|| Fragment Size(s) || Strain(s) || Notes
 |-
-|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] ||  || Two bands if heterozygote
+|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || aTSC, mTSC, Placenta TSC || Two bands if heterozygote
 |-
-|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
+|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || Between 340-400kDa || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR || Only present if Transgene Positive (cannot detect homozygosity at transgene)
 |-
-|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]||  || Two bands if heterozygote
+|[[SRY Genotyping| SRY]]|| [[Cre PCR ]] || Between 100-200kDa || any mice too young to sex visually || Only present in males
+|-
+|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]|| || || Two bands if heterozygote
 |-
-|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
+|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || || Two bands if heterozygote
+|-
+|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
 |-
-|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
+|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp  || || Separate Reactions for WT and KO
 |-
-|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575, WT-714  || Separate Reactions for WT and KO
+|[[CAP Genotyping|CAP]] || CAP ||  || || Two bands if heterozygote, upper band for KO
+|-
+|[[Vinexin Genotyping|Vinexin]] || Vinexin || || ||
+|-
+|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || GR-KO and Adipo-GR-KO || Two bands if heterozygote
+|-
+|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540bp, WT- 400bp || mc4r || Two bands if heterozygote (run primers separately)
+|-
+|[[ppp1r3c Genotyping| PTG]] || ppp1r3c (PTG) || KO- 574bp, WT- 678bp || ppp1r3c (PTG) || Two bands if heterozygote (run primers separately)
 |-
-|[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote, upper band for KO
+|[[4EBP1/2 Genotyping| 4EBP1/2]] || [[Cre PCR]] || 1= KO- 550bp, WT- 175bp; 2= KO- 750bp, WT- 450bp || 4EBP1/2 || Two bands if heterozygote for each allele (run primers for 1 and 2 separately)
 |-
-|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
+|[[GDF15 Genotyping| GDF15]] || GDF15 || KO- 200bp, WT- 600bp || GDF15 || Two bands if heterozygote (run primers separately)
 |}
+AMPK, fTSC, fRaptor, and C57Bl6/J are all wild-type mice and don't need to be genotyped.
 [[ Category:Genotyping ]]
 [[ Category:Mouse Work]]