Genotyping Program: Difference between revisions

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|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || aTSC, mTSC, Placenta TSC || Two bands if heterozygote

|-

|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR || Only present if Transgene Positive (cannot detect homozygosity at transgene)

|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || Between 340-400kDa || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR || Only present if Transgene Positive (cannot detect homozygosity at transgene)

|-

|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]|| || Two bands if heterozygote

|[[SRY Genotyping| SRY]]|| [[Cre PCR ]] || Between 100-200kDa || any mice too young to sex visually || Only present in males

|-

|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]|| || || Two bands if heterozygote

|-

|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote

|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || || Two bands if heterozygote

|-

|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]

|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]

|-

|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp || Separate Reactions for WT and KO

|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp || || Separate Reactions for WT and KO

|-

|[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote, upper band for KO

|[[CAP Genotyping|CAP]] || CAP || || || Two bands if heterozygote, upper band for KO

|-

|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||

|[[Vinexin Genotyping|Vinexin]] || Vinexin || || ||

|-

|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || GR-KO and Adipo-GR-KO || Two bands if heterozygote

@@ Line 4: / Line 4: @@
 |[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || aTSC, mTSC, Placenta TSC || Two bands if heterozygote
 |-
-|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR || Only present if Transgene Positive (cannot detect homozygosity at transgene)
+|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || Between 340-400kDa || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta TSC, Placenta GR || Only present if Transgene Positive (cannot detect homozygosity at transgene)
 |-
-|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]||  || Two bands if heterozygote
+|[[SRY Genotyping| SRY]]|| [[Cre PCR ]] || Between 100-200kDa || any mice too young to sex visually || Only present in males
+|-
+|[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]|| || || Two bands if heterozygote
 |-
-|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
+|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || || Two bands if heterozygote
 |-
-|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
+|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
 |-
-|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp  || Separate Reactions for WT and KO
+|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp  || || Separate Reactions for WT and KO
 |-
-|[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote, upper band for KO
+|[[CAP Genotyping|CAP]] || CAP ||  || || Two bands if heterozygote, upper band for KO
 |-
-|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
+|[[Vinexin Genotyping|Vinexin]] || Vinexin || || ||
 |-
 |[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || GR-KO and Adipo-GR-KO || Two bands if heterozygote