Tamoxifen Injections for Inducible Knockouts: Difference between revisions
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==SOP== | |||
*[[Safety and Animal Training]] | |||
*[[SOP - Needle Capping]] | |||
*[[SOP- Tamoxifen]] | |||
==METHODS== | |||
* Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)-- must wear nitrile gloves when dealing with tamoxifen. | * Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)-- must wear nitrile gloves when dealing with tamoxifen. | ||
** Tamoxifen takes a long time to go into solution so plan to prepare ahead of time. | |||
* Inject 50 mg/kg on five consecutive days, at the same time each day. The time is important, otherwise they will overdose if you inject too close together. | * Inject 50 mg/kg on five consecutive days, at the same time each day. The time is important, otherwise they will overdose if you inject too close together. | ||
* To determine how much to inject weigh the mouse and inject 5 uL/g of mouse. This means a 25 g mouse will get an injection of 125 uL. | * To determine how much to inject weigh the mouse and inject 5 uL/g of mouse. This means a 25 g mouse will get an injection of 125 uL. | ||
* Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles. | ** Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles. | ||
* Tamoxifen can be stored at room temperature for up to a week. | * Tamoxifen can be stored at room temperature for up to a week. | ||
* The initial weights can be used for the rest of the week when determining injection volume, unless there is reason to believe they will be changing drastically. | * The initial weights can be used for the rest of the week when determining injection volume, unless there is reason to believe they will be changing drastically. | ||