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Adipocyte Cell Counting with ImageJ

622 bytes added, 18:40, 31 March 2015
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****Select "MRI_Adipocyte_Tools" from downloads to install.
==Adipocyte Cell Counting==
*With macro installed, click on segmentation: "s"
**Set boundaries for sizing of cell
***Use 40-40,000 for size typically.
**Change thresholding method to Default.
*Open image of interest.
*Click on segmentation "s"
*Select freehanding tool to outline any cells that were not counted
**To add freehanded cell to ROI manager, click command + T
*Once all cells have been outlined, and misnumbered/outlined cells have been removed, click on measure
**Copy and save measurements to Excel spreadsheet
===To save image of outlined cells===
*Make sure all cells are outlined.
*Click on "Flatten"
*Save flattened image to folder.
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