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Undo revision 853 by Davebridges (talk)
==Protocol==
#Weigh out 30mg tissue (record weights for normalization) on dry ice into round bottom eppendorf tube. Add one stainless steel ball bearing.
#Add 200ul 500ul Homogenization Buffer#Homogenize by sonicating on ice with Qiagen Tissue Lyser for 3 x 15s minutes @ 25Hz for Liver/WAT or 3 x freeze thaws.for 5 minutes @ 30Hz for muscle#Add 5 ul 12.5ul KOH
#Mix by inverting
#Add 800ul '''Chloroform/Methanol Mixture'''
