903
edits
Changes
Updated protocol for tissue culture
==Protocol==
#Weigh out 30mg tissue (record weights for normalization) on dry ice into round bottom eppendorf tube. Add one stainless steel ball bearing.
#Add 500ul 200ul Homogenization Buffer#Homogenize with Qiagen Tissue Lyser for by sonicating on ice 3 minutes @ 25Hz for Liver/WAT x 15s or for 5 minutes @ 30Hz for muscle3 x freeze thaws.#Add 12.5ul 5 ul KOH
#Mix by inverting
#Add 800ul '''Chloroform/Methanol Mixture'''
