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Preparation of Tail Samples (for Genotyping)

31 bytes added, 17:18, 26 June 2013
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Protocol: Proteinase K
==Protocol==
# Combine 100-200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube or in a well of a 96 well PCR plage(Proteinase K stock = 10mg/mL)
# Incubate at 55 degrees (O/N)
# Incubate at 85 degrees for 45 min
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