Changes

# Combine 100-200uL 100uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf eppendorf tube or in a well of a 96 well PCR plage plate (Proteinase K stock = 10mg/mL)# Incubate at 55 degrees (16 hours - O/N)# Incubate at 85 degrees for 45 60 min # Run PCRHold at 4 degrees