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Generating and Amplifying Adenoviral Constructs

20 bytes added, 20:56, 11 July 2012
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# Add virus sample (up to 2.5 ml)
# Elute with 5 ml PBS, discard first 20 drops and collect 10 drop fractions into 10 tubes.
# OD 260 fractions in nanodrop, virus is in middle fractions (5-7 in my extraction). Good virus yield is >3 OD. # dilute to 1 OD/ml
# Add 10% glycerol and store at -80.
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