22
edits
Changes
no edit summary
# Add virus sample (up to 2.5 ml)
# Elute with 5 ml PBS, discard first 20 drops and collect 10 drop fractions into 10 tubes.
# OD 260 fractions in nanodrop, virus is in middle fractions (5-7 in my extraction). Good virus yield is >3 OD. # dilute to 1 OD/ml
# Add 10% glycerol and store at -80.