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→Purification of Adenovirus
#Add an equal volume of 2X Virus storage buffer and store at -80
#Titer the virus using a kit.
===desalting of CsCl purified adenovirus using PD-10 desalting columns (17-0851-01 GE Healthcare)===
# Cut column, discard storage solution and wash X5 with ~5ml PBS
# Add virus sample (up to 2.5 ml)
# Elute with 5 ml PBS, discard first 20 drops and collect 10 drop fractions into 10 tubes.
# OD 260 fractions in nanodrop, virus is in middle fractions (5-7 in my extraction). Good virus yield is >3 OD.
# Add 10% glycerol and store at -80.
===Tail Vein Injection===
# Insert mouse to restriction device. Carefully warm tail with lamp/warm water, inject 0.1 OD/mouse (0.15 for HFD mouse) using insulin syringe.
# Injected mice are housed in biohazard mouse room for 3 days (need bright-pink barcode stickers and water bottles).
# Adenovirus is detected in liver for at least 2-3 weeks, shRNA has maximal effect at 4-6 days, usually gone in 10 days.
