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==Materials==
*Instead of FM4-64 we use SynaptoRed (Sigma #S6689). This is resuspended 1mg to 500 uL (2ug/mL) with DMSO.and stored in '''Molecular Biology Stuff'''
*Media (see [[YPD Media and Agar]])
*Dye solution. For each cell line need 250 uL media + 3 uL dye.
