Changes

FM4-64 Labeling of Yeast Cells

56 bytes added, 19:40, 7 December 2009

added SynaptoRed concentration

==Materials==

*Instead of FM4-64 we use SynaptoRed (Sigma #S6689). This is resuspended 1mg to 500 uL (2ug/mL) with DMSO.

*Media (see [[YPD Media and Agar]])

*Dye solution. For each cell line need 250 uL media + 3 uL dye.

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