906
edits
Changes
Added details about ipGTT
#Remove food from mice for about 6h. Add do not feed tag to cages.
#Bring mice to procedure room. Put water bottle on cage.
#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capilary capillary about 2/3 full with blood.
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouseor the mouth if doing an oral glucose tolerance test.
##Immobilize mouse and restrain tail with one hand
##'''For ipGTT''' Aim needle between the midline and the hip bone##'''For oGTT''' Gently inert gavage needle into mouth##Insert syringe (do not inject) into cavity/mouth##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
##Eject syringe.
#At desired intervals (normally 15, 30, 45, 60, 75, 90 , and 120 min), take blood glucose measurements from the tail vein
[[Category:Mouse Work]]
[[Category:Glucose Homeostasis]]
[[Category:Injections]]
