164
edits
Changes
→PBND Solution: Tail Lysis Buffer
==PBND Solution: Tail Lysis Buffer==
* 50mM KCl (Hint: KCl is very heavy, you'll only need a tiny bit)
* 10mM Tris HCl (pH~8.3)
* 0.1 mg/mL MgCl2, 6H20
* 0.1 mg/mL Gelatin
* 0.45% (NP-40) Jgepal (Hint: Use cut tips, as NP-40 is very viscous)* 0.45% Tween 20 (Hint: Use cut tips, as Tween 20 is very viscous) PBDN can be made in liter volume, aliquoted into 50ml falcon tubes, frozen, and used as needed For 250ml PBND: * 0.930g 50mM KCl* 0.300g 10mM Tris HCl (pH~8.3)* 0.025g 0.1 mg/mL MgCl2, 6H20* 0.025g 0.1 mg/mL Gelatin* 1.125g 0.45% (NP-40) Jgepal * 1.125g 0.45% Tween 20 1. Start by adding 150ml ddH2O to a beaker on a stir plate (with magnetic stir bar).2. Add reagents. Mix well.3. Add 37% HCl drop-wise to adjust pH to 8.3.4. Add ddH20 to 250ml.
==Protocol==
