Changes

Jump to: navigation, search

Preparing Cell Lysates

117 bytes added, 21:14, 26 May 2009
edited table
==Materials==
===RIPA Buffer (for 10mL lysis buffer)==={border = "1" cellspacing = "0" cellpadding = "5"|-Tris pH7.4 !! 50mM !! 500uL|-Na Deoxycholate !! 0.25% !! 250uL|-NP-40 !! 1% !! 1mL|-NaCl !! 150mM !! 375uL|-EDTA !! 1mM !! 20uL|-NaVO3 !! 1mM !! 100uL|-NaF !! 1mM !! 20uL|-Protease Inhibitors !! 1 tab
Tris pH7.4 50mM 500uL Na Deoxycholate 0.25% 250uL NP-40 1% 1mL NaCl 150mM 371uL EDTA 1mM 20uL NaVO3 1mM 100uL NaF 1mM 20uL Protease Inhibitors 1 tab ==Basic Protocol==
# Stimulate cells if necessary
# Wash cells 2x1mL with ice cold PBS -/- and aspirate
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Special:MobileDiff/131"

Navigation menu