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Preparing Cell Lysates

8 bytes added, 15:42, 11 May 2009

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RIPA Buffer (for 10mL lysis buffer)

Tris pH7.4 50mM 500uL

Na Deoxycholate 0.25% 250uL

NP-40 1% 1mL

NaCl 150mM 371uL

EDTA 1mM 20uL

NaVO3 1mM 100uL

NaF 1mM 20uL

Protease Inhibitors 1 tab

5

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Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Special:MobileDiff/107"

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