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→Prepare Gel (BioRad Gel Rig)
==Prepare Gel (BioRad Gel Rig)==
#Place plastic mold securely in holder and turn handle to tighten#Put 15 or 8 well comb in mold as appropriate#Weight out 01.3g 0g agarose and add 30 100 mL of TAE(1% solution) then return TAE to shelf#Microwave 40s 90s until dissolved #Add 1 2 uL ethidium bromideGel Red. Swirl to mix and eliminate sediments
#Pour into mold
#Place gel in cold room if in a hurry
#After loading, run gel at 100V and dispose in ethidium bromid waste
