903
edits
Changes
updated with note about using less tissue
==Protocol==
# Weigh out 200-500mg tissue (record weight for normalization). You can use less tissue if necessary by reducing the lysis volume (must be greater than 500 uL) or increasing the amount of chloroform layer removed (normally 180 uL).
# Homogenize with dounce homogenizer in 2 mL '''Homogenization Buffer'''.
# Remove 200 uL to a tube containing 5 uL KOH
