Changes

# If absorbance is going to be measured by cuvette, use non-bolded values. If you are using a plate reader use bolded values.# Add 50uL '''(500uL)''' of '''Butanol Mixture'''# Measure triglyceride levels using Sigma Diagnostic Kit using 5 uL of sample:## Resuspend triglyceride and glycerol reagent with water if necessary.## Calculate how many samples you have (samples + buffer blank + 6 standard curve values).## Prepare reagent, you need 560 uL '''(80uL)''' of glycerol reagent and 140 uL '''(20uL)''' of triglyceride reagent. Make a bit extra and combine in a falcon tube.## Aliquot 700 uL into a cuvette or '''100 uL into a well of a 96 well plate'''.## For standards add 0-5 uL of glycerol standard '''(or of a 1/10 dilution of the glycerol standard)'''.## Add 5 uL of resuspended lipid to each well to start (also make a 5uL blank of the butanol mixture) and mix.## Let sit for ~30 min at room temperature (or 5 min at 37C if you are in a hurry).## Measure absorbance at 540 nm.## If any samples are A540<0.1 or above the 5uL standard A540 then repeat with more or less lipid as required.