Difference between revisions of "Transfer Buffer"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (copied recipe from saltiel lab buffers) |
m (→10X Transfer Buffer: Changed from 10L total to 4L total) |
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{| border="1" | {| border="1" | ||
| − | ! Component || Weight per | + | ! Component || Weight per 4 L || Catalog Number |
|- | |- | ||
| − | |Tris Base || | + | |Tris Base || 121.2 g || Sigma T6066-5KG |
|- | |- | ||
| − | |Glycine || | + | |Glycine || 576.4 g || Sigma G7126-5KG |
|} | |} | ||
[[ Category:Buffer ]] | [[ Category:Buffer ]] | ||
[[ Category:Western Blotting ]] | [[ Category:Western Blotting ]] | ||
Latest revision as of 19:34, 11 July 2013
10X Transfer Buffer
To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:
- 100 mL 10X Buffer
- 200 mL Methanol
- 700 mL Water
| Component | Weight per 4 L | Catalog Number |
|---|---|---|
| Tris Base | 121.2 g | Sigma T6066-5KG |
| Glycine | 576.4 g | Sigma G7126-5KG |
