Search results

Create the page "PCR" on this wiki! See also the search results found.

Page title matches

Page text matches

• Isolation of Mouse Embryonic Fibroblasts (MEFs)
  # Disect out all four limbs plus the tail. Place one small piece into PCR tube/plate for genotyping
  741 bytes (117 words) - 15:50, 5 September 2012
• Ob/ob Genotyping
  ==PCR Reaction== The PCR program is called '''ob-ob''' and is:
  1 KB (195 words) - 19:59, 26 September 2013
• 5' RACE
  <li> Combine the following in a PCR tube:</li> <li> Incubate at 70C for 10 min to denature RNA using the PCR Program '''5 RACE 70C'''</li>
  3 KB (551 words) - 21:04, 4 December 2012
• TSC-floxed Genotyping
  ...sc1 and the deleted allele using the following three primers in a 35 cycle PCR reaction using Perkin Elmer AmpliTaq Gold: F4536, 5′-AGGAGGCCTCTTCTGCTACC [[ Category:PCR]]
  1 KB (141 words) - 19:11, 30 December 2012
• MTM1 Genotyping
  [[ Category:PCR]] ==PCR Program==
  663 bytes (93 words) - 15:17, 21 January 2013
• TC10b Genotyping
  [[ Category:PCR]]
  495 bytes (59 words) - 14:55, 5 July 2013
• Cre PCR
  ==PCR Program== [[ Category:PCR]]
  301 bytes (43 words) - 18:42, 25 April 2018
• Primer Design for qPCR
  # Under PCR Product Size pick 70-150 as the range ...opriate primer pair in that region is not selected, change the range under PCR template on the top
  1 KB (249 words) - 17:48, 29 June 2017
• QPCR
  *SYBR Green PCR Master Mix Applied Biosystems (ThermoFisher Catalog # 4367659; [https://www
  4 KB (585 words) - 19:13, 8 November 2023
• Cloning CRISPR-Cas Plasmids
  * First digest vector by adding to a PCR tube (or use a frozen, pre-digested vector): * Next prepare the insert by annealing and phosphorylating the primers in a PCR tube:
  2 KB (324 words) - 19:41, 19 October 2017
• PCR Amplification with Platinum Taq High Fidelity
  * Platinum PCR SuperMix High Fidelity (Life Technologies cat# 12532-016) * The PCR program should be
  745 bytes (105 words) - 13:43, 17 July 2015
• TaqMan miRNA Assay
  ...w.lifetechnologies.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays.html * You will then prepare a tube for each primer/RNA set as such in a PCR tube:
  1 KB (234 words) - 18:52, 30 December 2014
• Surveyor Assay for Genomic DNA Mutations
  * Platinum PCR SuperMix High Fidelity (Life Technologies cat# 12532-016) * Place in PCR tube and digest using '''quick-extract''' PCR program:
  2 KB (262 words) - 20:02, 13 July 2015
• Quantification of miRNA by SYBR Green qPCR
  * Briefly centrifuge then transfer to a PCR tube and add (per reaction): * Mix gently by pipetting and place in the PCR machine for the following program (called '''SS III Reaction'''):
  3 KB (432 words) - 15:24, 1 March 2017
• TaqMan SNP Assay
  *StepOnePlus Real-Time PCR System (Applied Biosystems).
  1 KB (155 words) - 16:39, 16 November 2015
• SHROB Genotyping
  ...fragments of 82 bp and 39 bp in size (lanes 6-1 0). Tru9l digestion of the PCR products from heterozygous lean Koletsky (+/fa rats gave rise to three 121- [[ Category:PCR]]
  3 KB (371 words) - 17:09, 8 June 2020
• Chromatin Immunoprecipitation
  * QIAquick PCR Purification Kit 39. Add 5 volumes Qiagen Buffer PB (QIAquick PCR Purification Kit) to one volume of ChIP’d DNA. Add pH detector (at a 1:25
  11 KB (1,742 words) - 15:23, 10 May 2018
• RT-PCR primer design for ChIP
  # PCR product size should be set to 70-150bp. [[ Category: PCR ]]
  1 KB (224 words) - 14:24, 21 August 2016
• Locating ChIP-seq peaks from ENCODE
  # Design primers for genes you believe your protein is bound to [[RT-PCR primer design for ChIP]]
  1 KB (272 words) - 17:15, 25 January 2016
• Chromatin Immunoprecipitation for Tissue Samples
  # Add 5 volumes Qiagen Buffer PB (QIAquick PCR Purification Kit) to one volume of ChIP’d DNA. Add pH detector (at a 1:25 # Add half (~600 µl) of the solution to a QIAquick PCR Purification column, centrifuge for 30-60 sec @ 13,000 RPM , and then repea
  6 KB (1,077 words) - 21:43, 10 June 2016

View (previous 20 | next 20) (20 | 50 | 100 | 250 | 500)