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The following pages are not linked from or transcluded into other pages in Bridges Lab Protocols.

Showing below up to 50 results in range #51 to #100.

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  1. How to design primer pairs for determining the presence of CrispR-cas induced mutations
  2. Immobilizing a Protein on a CM5 Chip for SPR
  3. Immunoprecipitation
  4. Inositol Labelling of Yeast Cells
  5. Installing Software to a UNIX Server
  6. Isolation of Mouse Embryonic Fibroblasts (MEFs)
  7. Isolation of Single Fibers for Seahorse Assays
  8. Ketone Body Tolerance Test
  9. Lipid Transfection
  10. Lipofectamine Transfection of C2C12 Cells
  11. Lipolysis Assay from Primary Adipocytes
  12. Liposome Binding Assay
  13. Main Page
  14. Maintanence of 18C Fly Stocks
  15. Mammary Gland Fat and SVF Separation
  16. Matrigel Basement Membrane Matrix
  17. Measurement of Glycolysis and Respiration Using Seahorse
  18. Measuring Autophagy in Tissue Culture Cells
  19. Measuring Cross-Sectional Area (CSA)
  20. Measuring Grip Strength
  21. Measuring Lifespan in Drosophila
  22. Measuring Phagocytosis using pHrodo Bioparticles
  23. Milk Creamatocrit
  24. NADH NBT Staining
  25. Oil red o stain for in vitro adipogenesis
  26. Ordering Barcodes for Mice
  27. Ordering Prescriptions-University of Michigan
  28. Osmotic Minipump Implantation
  29. PAS Staining
  30. PCR Amplification of DNA (KOD Polymerase)
  31. PCR Amplification with Platinum Taq High Fidelity
  32. PEI Mediated Plasmid Transfection
  33. Parsing XSQ Files
  34. Performing Drosophila Crosses
  35. Phosphate Buffered Saline
  36. Phred Quality Scores
  37. Preparation of Mouse Muscle for Cryosectioning
  38. Preparation of Protein Lysates from Cells
  39. Preparation of RNA Sample from Cell Culture
  40. Preparation of Tail Samples (for Genotyping)
  41. Preparing and Sending Tissues to Histology Core
  42. Protein-Lipid Overlay Assay
  43. Purification of GST-HA-S6K
  44. Purification of GST Fusion Proteins by GSTrap
  45. Purification of MBP Fusion Proteins
  46. Pyruvate Tolerance Test
  47. Quantification of Vacuolar Colocalization
  48. Quantification of miRNA by SYBR Green qPCR
  49. Quantifying Relative Expression from qRT-PCR
  50. RNA-seq Pipeline for Known Transcripts

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