Difference between revisions of "RIPA Buffer"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (changed vanadate concentration and volume) |
(→RIPA Buffer (for 10mL lysis buffer)) |
||
| Line 17: | Line 17: | ||
|NaF || 5mM || 100uL || 500mM || Shelf | |NaF || 5mM || 100uL || 500mM || Shelf | ||
|- | |- | ||
| − | |NaPPi || | + | |NaPPi || 10 mM || 1 mL || 100mM || Shelf |
|- | |- | ||
| Protease Inhibitors || || 1 mini tablet || || 4C | | Protease Inhibitors || || 1 mini tablet || || 4C | ||
|} | |} | ||
[[ Category:Buffer ]] | [[ Category:Buffer ]] | ||
Revision as of 15:21, 7 August 2013
RIPA Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| Tris pH7.4 | 50mM | 500uL | 1M | Shelf |
| Na Deoxycholate | 0.25% | 250uL | 10% | Shelf |
| NP-40 | 1% | 1mL | 10% | Tingting |
| NaCl | 150mM | 375uL | 4M | Shelf |
| EDTA | 1mM | 20uL | 0.5M | Shelf |
| NaVO3 | 100uM | 10uL | 100mM | Biochemical Stocks (-20) |
| NaF | 5mM | 100uL | 500mM | Shelf |
| NaPPi | 10 mM | 1 mL | 100mM | Shelf |
| Protease Inhibitors | 1 mini tablet | 4C |
