Difference between revisions of "Preparing an Agarose Gel"
From Bridges Lab Protocols
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*Agarose | *Agarose | ||
*TAE Buffer (1X): Dilute from 100X stock | *TAE Buffer (1X): Dilute from 100X stock | ||
| − | * | + | *Thermo Apparatus (Mold, Holder, Comb and Gel Rig) |
==Prepare Gel (BioRad Gel Rig)== | ==Prepare Gel (BioRad Gel Rig)== | ||
Revision as of 19:19, 24 August 2016
Materials
- Agarose
- TAE Buffer (1X): Dilute from 100X stock
- Thermo Apparatus (Mold, Holder, Comb and Gel Rig)
Prepare Gel (BioRad Gel Rig)
- Place plastic mold in holder and turn handle to tighten
- Put 15 or 8 well comb in mold as appropriate
- Weight out 0.3g agarose and add 30 mL of TAE
- Microwave 40s until dissolved
- Add 1 uL ethidium bromide
- Pour into mold
- Place gel in cold room if in a hurry
- After loading, run gel at 100V and dispose in ethidium bromid waste
