Difference between revisions of "Main Page"
From Bridges Lab Protocols
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==Transcriptional Analysis== | ==Transcriptional Analysis== | ||
*[[Real Time PCR From Cell Culture]] | *[[Real Time PCR From Cell Culture]] | ||
| + | *[[Harvesting RNA from Cells grown in monolayer]] | ||
==Mouse Protocols== | ==Mouse Protocols== | ||
Revision as of 13:31, 13 May 2009
Contents
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Harvesting RNA from Cells grown in monolayer
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
