Difference between revisions of "Low Salt Immune Complex Wash Buffer"
From Bridges Lab Protocols
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! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location | ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location | ||
|- | |- | ||
| − | | | + | | SDS || 1% || 150uL || 10% || Solutions Shelf |
|- | |- | ||
| − | | | + | | Triton X-100 || 1% || 1.5mL || 10% || Solutions Shelf |
|- | |- | ||
| − | | | + | | NaCl || 150mM || 563uL || 4M || Solutions Shelf |
|- | |- | ||
| − | | EDTA || | + | | EDTA || 2mM || 60uL || 500mM || Solutions Shelf |
|- | |- | ||
| − | | Tris || | + | | Tris || 20mM || 300uL || 1M || Solutions Shelf |
|} | |} | ||
Latest revision as of 19:58, 9 January 2018
| Chemical | Final Concentration | Per 15 mL | Stock | Location |
|---|---|---|---|---|
| SDS | 1% | 150uL | 10% | Solutions Shelf |
| Triton X-100 | 1% | 1.5mL | 10% | Solutions Shelf |
| NaCl | 150mM | 563uL | 4M | Solutions Shelf |
| EDTA | 2mM | 60uL | 500mM | Solutions Shelf |
| Tris | 20mM | 300uL | 1M | Solutions Shelf |
