Difference between revisions of "High Fat Diet Treatment"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (filled in details about mouse diets) |
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| + | [[ Category: Diet ]] | ||
| + | [[ Category: Animal Studies ]] | ||
| + | [[ Category: Glucose Homeostasis ]] | ||
| + | [[ Category: Metabolism ]] | ||
| + | [[ Category: Mouse Work ]] | ||
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To place mice on a high fat diet, here is the general protocol: | To place mice on a high fat diet, here is the general protocol: | ||
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# Start when mice are 10 weeks of age. | # Start when mice are 10 weeks of age. | ||
Revision as of 14:53, 20 November 2012
To place mice on a high fat diet, here is the general protocol:
Diet Choices
For more information about diets see research diets
| Food | Vendor | Code | Caloric Content | Fat | Protein | Sucrose | Starch | Notes |
|---|---|---|---|---|---|---|---|---|
| Normal Chow Diet | Lab Diet | 5L0D | 3.02 kcal/g | 5% | 24% | 3.7% | 32% | Provided by ULAM |
| High Fat Diet | Research Diets | D12451 | 3.85 kcal/g | 45% | 20% | 17% | 7% | Pink |
| Control Diet (High Sucrose) | Research Diets | D12450B | 3.85 kcal/g | 10% | 20% | 35% | 31% | Yellow |
| Control Diet (High Starch) | Research Diets | D12450H | 3.85 kcal/g | 10% | 20% | 7% | 44% | Peach |
Treatment Protocol
- Start when mice are 10 weeks of age.
- For High Fat Diet mice place in cages 4 mice/cage as early as possible. Can us 5 mice/cage for normal chow/control diets.
- Re-cage (and ear tag mice if necessary) mice and replace food with ~400g food in the container. Record this weight.
- This food should last 2 weeks.
- Weigh mice at ~10 AM either weekly or bi-weekly and re-weigh food to determine food intake. Top up to 400g.
- Generally treatment lasts 12 weeks.
