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GoTaq PCR Genotyping

9 bytes added, 16:21, 10 August 2010

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==Materials~~(100uM)~~==

*GoTaq Master Mix

*Primer Mix(100uL Primers): Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20.

*Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template

*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.

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