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Created page with '==Materials(100uM)== *GoTaq Master Mix *Primer Mix: Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20. *Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, ...'
==Materials(100uM)==
*GoTaq Master Mix
*Primer Mix: Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20.
*Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template
*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.
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==Procedure==
*Prepare gel 30 minutes before PCR is finished.
*Prepare Reaction Mixture, adding 13x all materials except for the template.
*Add this mixture to each PCR tube.
*Label and add each template to the corresponding PCR tube.
*Run PCR under genotyping>inoki (~1 hour)
*Load samples in gel and run on 30V (horizontally.)
*GoTaq Master Mix
*Primer Mix: Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20.
*Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template
*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
==Procedure==
*Prepare gel 30 minutes before PCR is finished.
*Prepare Reaction Mixture, adding 13x all materials except for the template.
*Add this mixture to each PCR tube.
*Label and add each template to the corresponding PCR tube.
*Run PCR under genotyping>inoki (~1 hour)
*Load samples in gel and run on 30V (horizontally.)
