Difference between revisions of "Glucose Tolerance Test"
From Bridges Lab Protocols
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==Materials (Bring Downstairs)== | ==Materials (Bring Downstairs)== | ||
| − | *Glucometer | + | *Glucometer - AccuChek Advantage |
| − | *Glucose Test Strips | + | *Glucose Test Strips - AccuChek Comfort Curve |
*Scale | *Scale | ||
*Beaker for weighing mice | *Beaker for weighing mice | ||
*Syringes | *Syringes | ||
*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. | *10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. | ||
| + | *Timer | ||
==Protocol== | ==Protocol== | ||
#Remove food from mice for about 6h. Add do not feed tag to cages. | #Remove food from mice for about 6h. Add do not feed tag to cages. | ||
| − | #Bring mice to procedure room. | + | #Bring mice to procedure room. Put water bottle on cage. |
#Weigh mice, mark tails as necessary and take fasting glucose measurement. | #Weigh mice, mark tails as necessary and take fasting glucose measurement. | ||
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
Revision as of 15:07, 20 May 2009
Materials (Bring Downstairs)
- Glucometer - AccuChek Advantage
- Glucose Test Strips - AccuChek Comfort Curve
- Scale
- Beaker for weighing mice
- Syringes
- 10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
- Timer
Protocol
- Remove food from mice for about 6h. Add do not feed tag to cages.
- Bring mice to procedure room. Put water bottle on cage.
- Weigh mice, mark tails as necessary and take fasting glucose measurement.
- Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
- At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
- Immobilize mouse and restrain tail with one hand
- Aim needle between the midline and the hip bone
- Insert syringe (do not inject) into cavity
- Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
- Eject syringe.
- At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein
