Genotyping Program
From Bridges Lab Protocols
| Allele | Program | Fragment Size(s) | Notes |
|---|---|---|---|
| TSC-floxed | Inoki PCR | Two bands if heterozygote | |
| Cre | Inoki PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | |
| Vac14 | Inoki PCR | Two bands if heterozygote | |
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote |
| ob/ob Genotyping | ob/ob | KO - 123bp, WT-104kb, control 191 bp | Two reactions, see ob/ob Genotyping |
| MTM1 | MTM1 | Mutant-575, WT-714 | Separate Reactions for WT and KO |
| CAP | CAP | Two bands if heterozygote, upper band for KO | |
| Vinexin | Vinexin |
