Difference between revisions of "Genotyping Program"
From Bridges Lab Protocols
(added newer genotyping strains) |
(added mc4r-Cone) |
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!| Allele || Program|| Fragment Size(s) || Notes | !| Allele || Program|| Fragment Size(s) || Notes | ||
|- | |- | ||
| − | |[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || | + | |[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || Two bands if heterozygote |
|- | |- | ||
|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene) | |[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene) | ||
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|- | |- | ||
|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote | |[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote | ||
| − | | | + | | |
|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]] | |[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]] | ||
|- | |- | ||
| − | |[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant- | + | |[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp || Separate Reactions for WT and KO |
|- | |- | ||
|[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote, upper band for KO | |[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote, upper band for KO | ||
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|[[Vinexin Genotyping|Vinexin]] || Vinexin || || | |[[Vinexin Genotyping|Vinexin]] || Vinexin || || | ||
|- | |- | ||
| − | |[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- | + | |[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || Two bands if heterozygote |
| + | |- | ||
| + | |[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540, WT- 400 || Two bands if heterozygote (run primers separately | ||
|} | |} | ||
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[[ Category:Genotyping ]] | [[ Category:Genotyping ]] | ||
[[ Category:Mouse Work]] | [[ Category:Mouse Work]] | ||
Revision as of 15:21, 5 June 2020
| Allele | Program | Fragment Size(s) | Notes | |||||
|---|---|---|---|---|---|---|---|---|
| TSC-floxed | Cre PCR | KO- 368bp, WT- 295bp | Two bands if heterozygote | |||||
| Cre | Cre PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | ||||||
| Vac14 | Cre PCR | Two bands if heterozygote | ||||||
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote | ob/ob Genotyping | ob/ob | KO - 123bp, WT-104kb, control 191 bp | Two reactions, see ob/ob Genotyping | |
| MTM1 | MTM1 | Mutant-575bp, WT-714bp | Separate Reactions for WT and KO | |||||
| CAP | CAP | Two bands if heterozygote, upper band for KO | ||||||
| Vinexin | Vinexin | |||||||
| GR KO | nr3c1 | KO- 300bp, WT- 247bp | Two bands if heterozygote | |||||
| mc4r-Cone | mc4r | KO- 540, WT- 400 | Two bands if heterozygote (run primers separately |
