Difference between revisions of "Genotyping Program"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (added MTM1 program) |
Davebridges (Talk | contribs) (added link to Cre PCR program) |
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!| Allele || Program|| Fragment Size(s) || Notes | !| Allele || Program|| Fragment Size(s) || Notes | ||
|- | |- | ||
| − | |[[TSC-floxed Genotyping| TSC-floxed]]|| | + | |[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || || Two bands if heterozygote |
|- | |- | ||
| − | |[[Cre Genotyping|Cre]] || | + | |[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene) |
|- | |- | ||
| − | |[[Vac14 Genotyping|Vac14]] || | + | |[[Vac14 Genotyping|Vac14]] || [[Cre PCR]]|| || Two bands if heterozygote |
|- | |- | ||
|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote | |[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote | ||
Revision as of 14:34, 9 July 2013
| Allele | Program | Fragment Size(s) | Notes |
|---|---|---|---|
| TSC-floxed | Cre PCR | Two bands if heterozygote | |
| Cre | Cre PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | |
| Vac14 | Cre PCR | Two bands if heterozygote | |
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote |
| ob/ob Genotyping | ob/ob | KO - 123bp, WT-104kb, control 191 bp | Two reactions, see ob/ob Genotyping |
| MTM1 | MTM1 | Mutant-575, WT-714 | Separate Reactions for WT and KO |
| CAP | CAP | Two bands if heterozygote, upper band for KO | |
| Vinexin | Vinexin |
