Difference between revisions of "Genotyping Program"
From Bridges Lab Protocols
(added newer genotyping strains) |
|||
| Line 18: | Line 18: | ||
|[[Vinexin Genotyping|Vinexin]] || Vinexin || || | |[[Vinexin Genotyping|Vinexin]] || Vinexin || || | ||
|- | |- | ||
| − | |[[nr3c1 Genotyping| | + | |[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300, WT- 247 || Two bands if heterozygote |
|} | |} | ||
[[ Category:Genotyping ]] | [[ Category:Genotyping ]] | ||
Revision as of 14:46, 5 June 2020
| Allele | Program | Fragment Size(s) | Notes |
|---|---|---|---|
| TSC-floxed | Cre PCR | Two bands if heterozygote | |
| Cre | Cre PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | |
| Vac14 | Cre PCR | Two bands if heterozygote | |
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote |
| ob/ob Genotyping | ob/ob | KO - 123bp, WT-104kb, control 191 bp | Two reactions, see ob/ob Genotyping |
| MTM1 | MTM1 | Mutant-575, WT-714 | Separate Reactions for WT and KO |
| CAP | CAP | Two bands if heterozygote, upper band for KO | |
| Vinexin | Vinexin | ||
| GR KO | nr3c1 | KO- 300, WT- 247 | Two bands if heterozygote |
