Difference between revisions of "Genotyping Program"

From Bridges Lab Protocols
Jump to: navigation, search
(added link to Cre PCR program)
Line 17: Line 17:
 
|-
 
|-
 
|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
 
|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
 +
|-
 +
|[[nr3c1 Genotyping|
 
|}
 
|}
 
[[ Category:Genotyping ]]
 
[[ Category:Genotyping ]]

Revision as of 14:43, 5 June 2020

Allele Program Fragment Size(s) Notes
TSC-floxed Cre PCR Two bands if heterozygote
Cre Cre PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Cre PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote
ob/ob Genotyping ob/ob KO - 123bp, WT-104kb, control 191 bp Two reactions, see ob/ob Genotyping
MTM1 MTM1 Mutant-575, WT-714 Separate Reactions for WT and KO
CAP CAP Two bands if heterozygote, upper band for KO
Vinexin Vinexin
[[nr3c1 Genotyping|