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*BioRad Protein Assay Dye Reagent Concentrate cat#500-0006
*Disposable Plastic Cuvette
*0.1mg/mL BSA in H20 as standard
==Protocol==
Cuvette Bradford Assay
#Dilute reagent 5X in water, stable for 2-3 weeks
#Pipet 1 mL into disposable plastic cuvette
##Set dilution to be 1/vol (ie 0.1 for 10 uL)
##Blank then measure samples, absorbance must be less than 0.9
##Print (hit Recall, then enter, then print) and attach to experiment Protein Lysate Bradford Assay#Dilute reagent 5X in water, stable for 2-3 weeks#In a 96 well plate, dilute sample 20X (190ul H2O, 10ul Sample)--this dilution factor is tissue-dependent, only need to dilute fat ~5x#Add 5ul of 20x diluted sample to either 100ul or 200ul of Bradford reagent in well.##Prepare a standard curve by adding 0.0-3.0ul BSA std (1mg/ml) in increments of 0.5ul for 100 ul or 0-6ul BSA std in increments of 1ul for 200ul.#Run "Bradford Assay Protocol" on Plate Reader
==Reference==
*Wikipedia: [[wikipedia:Bradford_protein_assay|Bradford Protein Assay]]<pubmed>*PMID 942051</pubmed>
