164
edits
Changes
Adjusted bradford reagent vol and added standard curve details
#Dilute reagent 5X in water, stable for 2-3 weeks
#In a 96 well plate, dilute sample 20X (190ul H2O, 10ul Sample)--this dilution factor is tissue-dependent, only need to dilute fat ~5x
#Add 5ul of 20x diluted sample to either 100ul or 200ul of Bradford reagent in well.##Prepare a standard curve by adding 0.0-3.0ul BSA std (1mg/ml) in increments of 0.5ul for 100 ul or 0-6ul for 200ul.
#Run "Bradford Assay Protocol" on Plate Reader
