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• Preparation of Protein Lysates from Mouse Tissues
  #Follow Protein Lysate instructions for Bradford Assay (see [[Bradford Assay]]) ...2X loading buffer with B-ME to each lyaste. This will generate a 2 mg/mL protein solution in SDS Loading Buffer
  1 KB (253 words) - 16:06, 25 April 2019
• Affinity Purification of Proteins - FPLC
  #Check that the HPLC is set up for protein work and not radioactive work (see [[HPLC - Plumbing Setup]]). ##Place the protein lead into the equillibration buffer and prime.
  3 KB (559 words) - 19:47, 9 February 2011
• Statistical Analysis
  ...umn if there are two different variables in the group column. It will not work if there are more than 2 groups ...ps internally. For example this might be Normal Diet, High Fat Diet, High Protein Diet. Note that if you do this with just two groups, the result should be
  6 KB (1,029 words) - 18:10, 26 June 2012
• High Fat Diet Treatment
  [[ Category: Mouse Work ]] ! Protein
  2 KB (270 words) - 18:42, 28 November 2018
• Preparation of Orthovanadate Stocks
  [[ Category: Protein Work ]]
  830 bytes (127 words) - 14:29, 2 August 2017
• SOP - Cytotoxins and Antineoplastic Drugs
  ...r bind directly to genetic material in the cell nucleus or affect cellular protein synthesis. Cytotoxic drugs may not distinguish between normal and cancerous All hazardous drug work will be conducted in a chemical fume hood or Class II Biological Safety Cab
  9 KB (1,252 words) - 16:48, 6 June 2022
• Preparation of Protein Lysates from Cells
  [[Category:Protein]] [[Category:Mouse Work]]
  882 bytes (140 words) - 21:43, 5 January 2018
• Scanning and Analyzing Western Blots Using LiCor Odyssey
  ...yellow icon on desktop). Click on or create (using your last name) your '''Work Area'''. ...ing for total protein, dry membrane if scanning finished blot) on scanner, protein side down. Cover with clear rubber mat and roll with roller to remove any
  5 KB (873 words) - 15:51, 26 March 2021