Chromatin Immunoprecipitation: Difference between revisions
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==== Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads==== | ==== Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads==== | ||
# Add 20 μl re-suspended magnetic bead slurry (for each sample you plan to use with this antibody) to a 1.5 ml microfuge tube on ice containing 1 ml cold PBS/BSA. | # Add 20 μl re-suspended magnetic bead slurry (for each sample you plan to use with this antibody) to a 1.5 ml microfuge tube on ice containing 1 ml cold PBS/BSA. Mix well, '''do not vortex'''. | ||
# Place the microfuge tubes on the magnet rack and remove supernatants. | # Place the microfuge tubes on the magnet rack and remove supernatants. | ||
# Resuspend the beads in 1 ml cold PBS/BSA. | # Resuspend the beads in 1 ml cold PBS/BSA. | ||