162
edits
Changes
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
# Add 200 μl PBS/BSA (for each sample you plan to use this antibody with) to beads.
# Add 1 μg primary antibody. Mix gently by tapping--Do not vortex beads after adding the antibody.
# Gently mix on a rotator platform for at least '''2 hours ''' at 4°C.
# Wash beads 3 times (steps b-c), resuspending the beads by inverting the tubes during each wash.
# Resuspend in 100 μl PBS/BSA (for each sample you plan to use this antibody with), and proceed to Step 2.
