==Protocol==
Use the following Volumes per 50ul 25ul Reaction:
Per sample 1X#10X GoTaq BufferDream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer)
#Primer Mix: 5ul
#dNTPs: 0.5uL of 10 mM ("Molecular Biology Stuff" box in freezer)#Sterile waterddH2O: 29ul #Polymerase Go-Taq: 07.125uL ("Molecular Biology Stuff" box in freezer)#Template: 1 uL5ul
Master Mix (Per 5mL -- Make 1mL Aliquots)#10X GoTaq Buffer: 625uL ("Molecular Biology Stuff" box in freezer) #Primer Mix: 625ul#dNTPs: 62.5uL of 10 mM ("Molecular Biology Stuff" box in freezer)#Sterile water: 3625ul #Polymerase Go-Taq: 15.625ul ("Molecular Biology Stuff" box in freezer)*Add Template Individually : 1 uL
Run PCR Program (approx 2 hours).
Use Cycler 1 on 6th Floor
*Login: Sergey, Just press enter to Login
*Under Genotype folder, pick Ingles program for Ingles genotyping
*Under Genotype folder, pick regpcr program for PLT genotyping
Make sure Run "specfic" PCR Program for gene of interest (approx 2 hours).* specific to press enter 2x once to confirm Tubes and second time to start PCReach gene
see [[Preparing an Agarose Gel]] for details on preparing a DNA gel
