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RIPA Buffer (for 10mL lysis buffer)
|
Final Concentration |
per 10 mL |
Stock |
Location
|
| Tris pH7.4 |
50mM |
500uL |
1M |
Chemical Shelf
|
| Na Deoxycholate |
0.25% |
250uL |
10% |
Chemical Shelf
|
| NP-40 |
1% |
1mL |
10% |
Chemical Shelf
|
| NaCl |
150mM |
375uL |
4M |
Shelf
|
| EDTA |
1mM |
20uL |
0.5M |
Shelf
|
| NaVO3 |
100uM |
10uL |
100mM |
Chemical Shelf
|
| NaF |
5mM |
100uL |
500mM |
Chemical Shelf
|
| NaPPi |
10 mM |
1 mL |
100mM |
Chemical Shelf
|
| Protease Inhibitors |
|
1 mini tablet |
|
4C
|