Triglyceride Assay from Drosophila (German Method)

Protocol

1. Use 5 female or 8 Male flies for Assay
2. Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock)
3. Heat samples in 70C waterbath for 5 minutes
4. Spin samples @ 5000G for 1 minute
5. Transfer 500ul of supernatent in new microfuge tube
6. Spin @ 14000G for 3 minutes
7. Add 50ul of sample to 200ul of TG solution in a 96 well plate
8. Incubate plate @ 37C for 5 mins
9. Measure 540nm absorbance

Publication of Method

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023796