Triglyceride Assay from Drosophila (German Method)

From Bridges Lab Protocols
Revision as of 21:12, 31 July 2013 by Mpeloqu1 (Talk | contribs) (Created page with "==Protocol== #Use 5 female or 8 Male flies for Assay #Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock) #Heat samples in 70C waterbath for 5 minutes #...")

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Protocol

  1. Use 5 female or 8 Male flies for Assay
  2. Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock)
  3. Heat samples in 70C waterbath for 5 minutes
  4. Spin samples @ 5000G for 1 minute
  5. Transfer 500ul of supernatent in new microfuge tube
  6. Spin @ 14000G for 3 minutes
  7. Add 50ul of sample to 200ul of TG solution in a 96 well plate
  8. Incubate plate @ 37C for 5 mins
  9. Measure 540nm absorbance
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php?title=Triglyceride_Assay_from_Drosophila_(German_Method)&oldid=777"