Submitting Plasmids for Sequencing

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Revision as of 19:26, 19 October 2017 by Iharvey (Talk | contribs) (Created page with "*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul *These should be submitted in 1.5mL tubes *Go to https://client-seqcore.brcf.med.um...")

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  • Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul
  • These should be submitted in 1.5mL tubes
  • Go to https://client-seqcore.brcf.med.umich.edu/login.cgi?exp=1
    • PI login: Bridges
    • Dave must add you to the lab under his account, he will give you your username and the password needed
  • Typically we will select Sanger Sequencing (the only low throughput option)
  • Select 'Submit samples/plates'
  • Select the appropriate short-code from the drop down menu at the top of this page
  • Generally choose 'One-at-a-time Sample Submission' unless you have a lot
  • From here enter the following for each section:
    • The sample name (the sample name that mean something to you regarding the sequence)
    • No discounts
    • Mini-Prep Plasmid
    • Whatever vector you used for this plasmid
    • Size of the insert you are sequencing
    • Custom Primers
    • Do not save
    • Do not rush
  • Save and repeat with your other samples
  • Go to ... for locations and drop off times