User contributions for Davebrid

15 July 2009

• 15:0815:08, 15 July 2009 diff hist +502 N Buffer/CHAPS Lysis Buffer wrote initial page

14 July 2009

• 18:4218:42, 14 July 2009 diff hist +66 Differentiation of 3T3-L1 Cells No edit summary

13 July 2009

• 14:1714:17, 13 July 2009 diff hist +249 N Monitoring Food Intake wrote initial page
• 13:4013:40, 13 July 2009 diff hist +29 m Main Page →Metabolic Measurements

10 July 2009

• 21:3121:31, 10 July 2009 diff hist 0 Measuring Fasting Insulin No edit summary

9 July 2009

• 16:5916:59, 9 July 2009 diff hist 0 Generation of Figures in Illustrator No edit summary
• 11:4711:47, 9 July 2009 diff hist +549 Real Time PCR From Cell Culture →RT-PCR Reaction: added details about RT Reaction

8 July 2009

• 15:5115:51, 8 July 2009 diff hist +37 Bradford Assay →Protocol: added note about printing
• 13:3313:33, 8 July 2009 diff hist +59 Buffer/RIPA mistake with NaF and NaPPi concentrations. Added note about NaVO3 location
• 13:2513:25, 8 July 2009 diff hist +1,184 N Preparation of RNA Samples from Mouse Tissues wrote initial page
• 13:0813:08, 8 July 2009 diff hist +24 m Preparation of Protein Lysates from Mouse Tissues No edit summary
• 13:0713:07, 8 July 2009 diff hist +836 N Preparation of Protein Lysates from Mouse Tissues wrote initial page
• 12:5812:58, 8 July 2009 diff hist +158 m Main Page →Mouse Protocols

1 July 2009

• 18:0518:05, 1 July 2009 diff hist +38 m Main Page →Cell and Tissue Culture
• 18:0518:05, 1 July 2009 diff hist +3,677 N Lipofectamine Mediated Knockdown copied initial page

30 June 2009

• 19:2119:21, 30 June 2009 diff hist 0 m Main Page →Protein Analysis
• 19:1819:18, 30 June 2009 diff hist 0 m Culturing RAW 264.7 Cells No edit summary
• 19:1719:17, 30 June 2009 diff hist 0 m Culturing RAW 264.7 Cells No edit summary
• 19:1519:15, 30 June 2009 diff hist +111 Splitting Cells →Cell Specific Notes
• 19:1419:14, 30 June 2009 diff hist +5,702 N Culturing RAW 264.7 Cells wrote initial page
• 19:1019:10, 30 June 2009 diff hist +106 Splitting Cells →Protocol

16 June 2009

• 14:4614:46, 16 June 2009 diff hist +31 m Main Page →Metabolic Measurements
• 14:4614:46, 16 June 2009 diff hist +321 N Measuring Fasting Insulin initial page
• 14:4314:43, 16 June 2009 diff hist +125 Glucose Tolerance Test added link to fasting insulin

10 June 2009

• 15:3215:32, 10 June 2009 diff hist +1 m Main Page No edit summary
• 14:2414:24, 10 June 2009 diff hist +1,032 N Talk:PCR Amplification of DNA comment from DGT current
• 13:4513:45, 10 June 2009 diff hist +1,286 N Insulin Tolerance Test initial page
• 13:4213:42, 10 June 2009 diff hist +28 m Main Page →Metabolic Measurements
• 13:4113:41, 10 June 2009 diff hist +189 Glucose Tolerance Test added glucose strip ordering information

8 June 2009

• 15:4315:43, 8 June 2009 diff hist +29 Generation of Figures in Illustrator No edit summary
• 15:4015:40, 8 June 2009 diff hist +196 Generation of Figures in Illustrator notes from excel
• 14:4114:41, 8 June 2009 diff hist +85 m Main Page No edit summary
• 14:4114:41, 8 June 2009 diff hist +107 N Generation of Figures in Illustrator first page
• 14:3514:35, 8 June 2009 diff hist +6 m Fugene Transfection of 293T/COS Cells No edit summary
• 14:3414:34, 8 June 2009 diff hist +706 N Fugene Transfection of 293T/COS Cells wrote initial page
• 14:2914:29, 8 June 2009 diff hist +43 m Main Page →Cell and Tissue Culture

7 June 2009

• 21:3221:32, 7 June 2009 diff hist 0 Electroporation of 3T3-L1 Adipocytes changed voltage to 160V

5 June 2009

• 18:0218:02, 5 June 2009 diff hist +3 m PCR Analysis of Tail DNA No edit summary
• 17:5917:59, 5 June 2009 diff hist −4 m PCR Analysis of Tail DNA No edit summary
• 17:5817:58, 5 June 2009 diff hist −23 m DNA Preparation from Tail Clip No edit summary

3 June 2009

• 23:5523:55, 3 June 2009 diff hist +61 Immunofluoresence →Protocol: added link

2 June 2009

• 20:0220:02, 2 June 2009 diff hist 0 Luciferase Assay No edit summary
• 20:0220:02, 2 June 2009 diff hist +1,256 N Luciferase Assay initial page
• 20:0120:01, 2 June 2009 diff hist +22 Main Page →Cell and Tissue Culture
• 17:1817:18, 2 June 2009 diff hist +39 Immunofluoresence →Protocol

28 May 2009

• 12:1312:13, 28 May 2009 diff hist +123 QPCR - Lin Lab →Before the run:: modified machine setup current
• 12:0912:09, 28 May 2009 diff hist −2 m Real Time PCR From Cell Culture →Plate Preparation

27 May 2009

• 22:0222:02, 27 May 2009 diff hist −33 m QPCR - Lin Lab →PCR Program:
• 21:5821:58, 27 May 2009 diff hist +42 m QPCR - Lin Lab →PCR Program:
• 21:5521:55, 27 May 2009 diff hist +13 m QPCR - Lin Lab →PCR Program: