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→Crosslinking, Lysis and Shearing of DNA
2. Add formaldehyde to a final concentration of 1% directly to the media of adherent cells growing on tissue culture plates, swirl gently, and
incubate at room temperature for 10 minutes (If using 10cm dishes add 0.5mL of the 2.5M glycerol glycine stock solution).
3. Stop the cross-linking reaction by adding glycine to a final concentration of 0.125M and swirl gently to mix.